Characterization and molecular cloning of the lectin from Helianthus tuberosus.

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  • Additional Information
    • Subject Terms:
    • Author-Supplied Keywords:
      Asteraceae
      cDNA cloning
      Helianthus tuberosus
      Jerusalem artichoke
      lectin
    • Abstract:
      A lectin called Helianthus tuberosus agglutinin or Heltuba has been isolated from tubers of the Jerusalem artichoke, a typical representative of the Asteraceae family. Heltuba is a tetrameric protein composed of four identical subunits of 15.5 kDa and exhibits a preferential specificity towards oligomannosides. Cloning of the corresponding cDNAs revealed that the mature lectin polypeptide comprises the entire open reading frame of the cDNA suggesting that the primary translation product is not processed and that the lectin is a cytosolic protein. Searches in the databases revealed sequence similarity with lectins from the taxonomically unrelated Convolvulaceae and Moraceae species. Therefore, the discovery of Heltuba is of great importance in view of the occurrence and molecular evolution of the jacalin‐related lectins. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of European Journal of Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      0014-2956
    • Accession Number:
      10.1046/j.1432-1327.1999.00013.x
    • Accession Number:
      5643189
  • Citations
    • ABNT:
      VAN DAMME, E. J. M. et al. Characterization and molecular cloning of the lectin from Helianthus tuberosus. European Journal of Biochemistry, [s. l.], v. 259, n. 1/2, p. 135–142, 1999. DOI 10.1046/j.1432-1327.1999.00013.x. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=5643189. Acesso em: 21 out. 2020.
    • AMA:
      Van Damme EJM, Barre A, Mazard A, et al. Characterization and molecular cloning of the lectin from Helianthus tuberosus. European Journal of Biochemistry. 1999;259(1/2):135-142. doi:10.1046/j.1432-1327.1999.00013.x
    • APA:
      Van Damme, E. J. M., Barre, A., Mazard, A., Verhaert, P., Horman, A., Debray, H., Rouge, P., & Peumans, W. J. (1999). Characterization and molecular cloning of the lectin from Helianthus tuberosus. European Journal of Biochemistry, 259(1/2), 135–142. https://doi.org/10.1046/j.1432-1327.1999.00013.x
    • Chicago/Turabian: Author-Date:
      Van Damme, Els J. M., Annick Barre, Anne‐Marie Mazard, Peter Verhaert, Alistair Horman, Henri Debray, Pierre Rouge, and Willy J. Peumans. 1999. “Characterization and Molecular Cloning of the Lectin from Helianthus Tuberosus.” European Journal of Biochemistry 259 (1/2): 135–42. doi:10.1046/j.1432-1327.1999.00013.x.
    • Harvard:
      Van Damme, E. J. M. et al. (1999) ‘Characterization and molecular cloning of the lectin from Helianthus tuberosus’, European Journal of Biochemistry, 259(1/2), pp. 135–142. doi: 10.1046/j.1432-1327.1999.00013.x.
    • Harvard: Australian:
      Van Damme, EJM, Barre, A, Mazard, A, Verhaert, P, Horman, A, Debray, H, Rouge, P & Peumans, WJ 1999, ‘Characterization and molecular cloning of the lectin from Helianthus tuberosus’, European Journal of Biochemistry, vol. 259, no. 1/2, pp. 135–142, viewed 21 October 2020, .
    • MLA:
      Van Damme, Els J. M., et al. “Characterization and Molecular Cloning of the Lectin from Helianthus Tuberosus.” European Journal of Biochemistry, vol. 259, no. 1/2, Jan. 1999, pp. 135–142. EBSCOhost, doi:10.1046/j.1432-1327.1999.00013.x.
    • Chicago/Turabian: Humanities:
      Van Damme, Els J. M., Annick Barre, Anne‐Marie Mazard, Peter Verhaert, Alistair Horman, Henri Debray, Pierre Rouge, and Willy J. Peumans. “Characterization and Molecular Cloning of the Lectin from Helianthus Tuberosus.” European Journal of Biochemistry 259, no. 1/2 (January 1999): 135–42. doi:10.1046/j.1432-1327.1999.00013.x.
    • Vancouver/ICMJE:
      Van Damme EJM, Barre A, Mazard A, Verhaert P, Horman A, Debray H, et al. Characterization and molecular cloning of the lectin from Helianthus tuberosus. European Journal of Biochemistry [Internet]. 1999 Jan [cited 2020 Oct 21];259(1/2):135–42. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=5643189