Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division.

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  • Author(s): Fraser, Ronald S.S.
  • Source:
    European Journal of Biochemistry. Jan75 Part 2, Vol. 50 Issue 3, p529-537. 9p.
  • Document Type:
    Article
  • Additional Information
    • Subject Terms:
    • Abstract:
      Messenger and ribosomal RNA metabolism was studied in a plant tissue culture system: cells from the quiescent tubers of Helianthus tuberosus (Jerusalem artichoke) were induced to divide synchronously and dedifferentiate by excision and culture of explants in nutrient medium. Large accumulations of ribosomal RNA and protein started early in the 20-h lag-period preceding the first division. In pulse-labelling experiments, two types of polydisperse messenger-like RNAs were detected, one with and one without a poly(adenylic acid) sequence. In the first 2 h of culture the two polydisperse RNA fractions were the predominant types of RNA synthesised. Ribosomal RNA synthesis was very low during the first 2 h, but accelerated later. Low concentrations of actinomycin-D strongly inhibited ribosomal RNA synthesis, but had little effect on the synthesis of transfer RNA or either type of polydisperse messenger-like RNA. In explants cultured with low concentrations of actinomycin-D ribosomal RNA accumulation was completely inhibited, but cell division and protein accumulation occurred, though at a reduced rate. It is concluded that the synthesis and accumulation of new ribosomal RNA which normally occur during culture are not required for the induction of cell division or for protein accumulation, i.e. the ribosomal RNA existing in the quiescent tuber cells can support protein accumulation and cell division induced by excision and culture. The quiescent tuber tissue is also shown to contain significant amounts of messenger-like RNA. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of European Journal of Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      0014-2956
    • Accession Number:
      10.1111/j.1432-1033.1975.tb09893.x
    • Accession Number:
      12606086
  • Citations
    • ABNT:
      FRASER, R. S. S. Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division. European Journal of Biochemistry, [s. l.], v. 50, n. 3, p. 529–537, 1975. DOI 10.1111/j.1432-1033.1975.tb09893.x. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=12606086. Acesso em: 2 dez. 2020.
    • AMA:
      Fraser RSS. Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division. European Journal of Biochemistry. 1975;50(3):529-537. doi:10.1111/j.1432-1033.1975.tb09893.x
    • APA:
      Fraser, R. S. S. (1975). Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division. European Journal of Biochemistry, 50(3), 529–537. https://doi.org/10.1111/j.1432-1033.1975.tb09893.x
    • Chicago/Turabian: Author-Date:
      Fraser, Ronald S.S. 1975. “Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division.” European Journal of Biochemistry 50 (3): 529–37. doi:10.1111/j.1432-1033.1975.tb09893.x.
    • Harvard:
      Fraser, R. S. S. (1975) ‘Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division’, European Journal of Biochemistry, 50(3), pp. 529–537. doi: 10.1111/j.1432-1033.1975.tb09893.x.
    • Harvard: Australian:
      Fraser, RSS 1975, ‘Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division’, European Journal of Biochemistry, vol. 50, no. 3, pp. 529–537, viewed 2 December 2020, .
    • MLA:
      Fraser, Ronald S. S. “Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division.” European Journal of Biochemistry, vol. 50, no. 3, Jan. 1975, pp. 529–537. EBSCOhost, doi:10.1111/j.1432-1033.1975.tb09893.x.
    • Chicago/Turabian: Humanities:
      Fraser, Ronald S.S. “Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division.” European Journal of Biochemistry 50, no. 3 (January 15, 1975): 529–37. doi:10.1111/j.1432-1033.1975.tb09893.x.
    • Vancouver/ICMJE:
      Fraser RSS. Studies on Messenger and Ribosomal RNA Synthesis in Plant Tissue Cultures Induced to Undergo Synchronous Cell Division. European Journal of Biochemistry [Internet]. 1975 Jan 15 [cited 2020 Dec 2];50(3):529–37. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=12606086