The Oxidation of Malate by Isolated Plant Mitochondria.

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  • Additional Information
    • Subject Terms:
    • Abstract:
      The functional relationship between NAD+-linked oxidations of L-malate and the operation of the exogenous and endogenous NADH dehydrogenases of isolated plant mitoehondria have been investigated. 1. Mitochondria isolated from tubers of the Jerusalem artichoke (Helianthus tuberosus) oxidised L-malate via an electron transport pathway which was inhibited by rotenone, piericidin A and added oxaloacetate. Phosphorylation linked to this pathway yielded an APD/O ratio of approx. 3. 2. In the presence of rotenone, piericidin A or added oxaloacetate, malate was oxidised by a pathway which was dependent on the presence of added NAD+ and low levels of Ca2+. Phosphorylation linked to this oxidation had an ADP/O ratio of approx. 2. 3. Oxidation of malate via the rotenone-sensitive pathway was inhibited by 2-n-butylmalonate whereas oxidation via the rotenone-insensitive pathway was unaffected by this compound. 4. By following the reduction of ferricyanide by malate in intact mitochondria, it was possible to identify two separate malate: NAD oxidoreductases: one located outside the inner membrane permeability barrier, the other inside this barrier. 5. Oxaloacetate was the primary product when malate was oxidised by the enzyme in the inner compartment which was therefore identified as L-malate: NAD oxidoreductase whereas pyruvate was the primary product of malate oxidation by the enzyme in the outer compartment, which was identified as L-malate : NAD oxidoreductase (decarboxylating) or malie enzyme. 6. The malic enzyme was extracted and partially purified from isolated mitochondria. The isolated enzyme showed a requirement for Mn2+ or Mg2+, required NAD+ as coenzyme, although activity to a lesser extent was obtained with NADP+. and was inhibited by NADH. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of European Journal of Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      0014-2956
    • Accession Number:
      10.1111/j.1432-1033.1972.tb01792.x
    • Accession Number:
      12465105
  • Citations
    • ABNT:
      COLEMAN, J. O. P.; PALMER, J. M. The Oxidation of Malate by Isolated Plant Mitochondria. European Journal of Biochemistry, [s. l.], v. 26, n. 4, p. 499–509, 1972. DOI 10.1111/j.1432-1033.1972.tb01792.x. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=12465105. Acesso em: 2 dez. 2020.
    • AMA:
      Coleman JOP, Palmer JM. The Oxidation of Malate by Isolated Plant Mitochondria. European Journal of Biochemistry. 1972;26(4):499-509. doi:10.1111/j.1432-1033.1972.tb01792.x
    • APA:
      Coleman, J. O. P., & Palmer, J. M. (1972). The Oxidation of Malate by Isolated Plant Mitochondria. European Journal of Biochemistry, 26(4), 499–509. https://doi.org/10.1111/j.1432-1033.1972.tb01792.x
    • Chicago/Turabian: Author-Date:
      Coleman, Julian O. P., and John M. Palmer. 1972. “The Oxidation of Malate by Isolated Plant Mitochondria.” European Journal of Biochemistry 26 (4): 499–509. doi:10.1111/j.1432-1033.1972.tb01792.x.
    • Harvard:
      Coleman, J. O. P. and Palmer, J. M. (1972) ‘The Oxidation of Malate by Isolated Plant Mitochondria’, European Journal of Biochemistry, 26(4), pp. 499–509. doi: 10.1111/j.1432-1033.1972.tb01792.x.
    • Harvard: Australian:
      Coleman, JOP & Palmer, JM 1972, ‘The Oxidation of Malate by Isolated Plant Mitochondria’, European Journal of Biochemistry, vol. 26, no. 4, pp. 499–509, viewed 2 December 2020, .
    • MLA:
      Coleman, Julian O. P., and John M. Palmer. “The Oxidation of Malate by Isolated Plant Mitochondria.” European Journal of Biochemistry, vol. 26, no. 4, Apr. 1972, pp. 499–509. EBSCOhost, doi:10.1111/j.1432-1033.1972.tb01792.x.
    • Chicago/Turabian: Humanities:
      Coleman, Julian O. P., and John M. Palmer. “The Oxidation of Malate by Isolated Plant Mitochondria.” European Journal of Biochemistry 26, no. 4 (April 1972): 499–509. doi:10.1111/j.1432-1033.1972.tb01792.x.
    • Vancouver/ICMJE:
      Coleman JOP, Palmer JM. The Oxidation of Malate by Isolated Plant Mitochondria. European Journal of Biochemistry [Internet]. 1972 Apr [cited 2020 Dec 2];26(4):499–509. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=12465105