Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast.

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    • Subject Terms:
    • Abstract:
      The catalytic properties of CYP73, cinnamate 4-hydroxylase isolated from Helianthus tuberosus tuber [Teutsch. H. G., Hasenfratz. M. P., Lesot. A., Stoltz. C., Garnier. J. M., Jeltsch. J. M., Durst. F. & Werck-Reichhart. D. (1993) Proc. Natl Acad. Sci. USA 90, 4102-4106] and expressed in an optimized yeast system [Urban. P., Werck-Reichhart. D., Teutsch. G. H., Durst F., Regnier. S., Kazmaier. M. & Pompon. D., (1994) Eur. J. Biochem. 222, 843-850] have been investigated. Microsomes from transformed yeast catalysed trans-cinnamate hydroxylation with high efficiency. CYP73 was highly specific for its natural substrate, and did not catalyse oxygenation of p-courmarate, benzoate, ferulate, naringenin or furanocoumarins. No metabolism to terpenoids or fatty acids, known substrates of plant P450s, was observed. CYP73 however demethylated the natural coumarin herniarin into umbelliferone. In addition, it was shown to oxygenate five xenobiotics and mechanism-based inactivators, including the herbicide chlorotoluron. All substrates of CYP73 were small planar aromatic molecules. Comparison of the kinetic parameters of CYP73 for its various substrates showed that, as expected, cinnamate was by far the best substrate of this P450. The physiological and toxicological significance of these observation are discussed. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of European Journal of Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • ISSN:
      0014-2956
    • Accession Number:
      10.1111/j.1432-1033.1994.00835.x
    • Accession Number:
      12357405
  • Citations
    • ABNT:
      PIERREL, M. A. et al. Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast. European Journal of Biochemistry, [s. l.], v. 224, n. 3, p. 835–844, 1994. DOI 10.1111/j.1432-1033.1994.00835.x. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=12357405. Acesso em: 2 dez. 2020.
    • AMA:
      Pierrel MA, Batard Y, Kazmaier M, Mignotte-Vieux C, Durst F, Werck-Reichhart D. Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast. European Journal of Biochemistry. 1994;224(3):835-844. doi:10.1111/j.1432-1033.1994.00835.x
    • APA:
      Pierrel, M. A., Batard, Y., Kazmaier, M., Mignotte-Vieux, C., Durst, F., & Werck-Reichhart, D. (1994). Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast. European Journal of Biochemistry, 224(3), 835–844. https://doi.org/10.1111/j.1432-1033.1994.00835.x
    • Chicago/Turabian: Author-Date:
      Pierrel, Marie Agnès, Yannick Batard, Michael Kazmaier, Claudia Mignotte-Vieux, Francis Durst, and Daniele Werck-Reichhart. 1994. “Catalytic Properties of the Plant Cytochrome P450 CYP73 Expressed in Yeast.” European Journal of Biochemistry 224 (3): 835–44. doi:10.1111/j.1432-1033.1994.00835.x.
    • Harvard:
      Pierrel, M. A. et al. (1994) ‘Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast’, European Journal of Biochemistry, 224(3), pp. 835–844. doi: 10.1111/j.1432-1033.1994.00835.x.
    • Harvard: Australian:
      Pierrel, MA, Batard, Y, Kazmaier, M, Mignotte-Vieux, C, Durst, F & Werck-Reichhart, D 1994, ‘Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast’, European Journal of Biochemistry, vol. 224, no. 3, pp. 835–844, viewed 2 December 2020, .
    • MLA:
      Pierrel, Marie Agnès, et al. “Catalytic Properties of the Plant Cytochrome P450 CYP73 Expressed in Yeast.” European Journal of Biochemistry, vol. 224, no. 3, Sept. 1994, pp. 835–844. EBSCOhost, doi:10.1111/j.1432-1033.1994.00835.x.
    • Chicago/Turabian: Humanities:
      Pierrel, Marie Agnès, Yannick Batard, Michael Kazmaier, Claudia Mignotte-Vieux, Francis Durst, and Daniele Werck-Reichhart. “Catalytic Properties of the Plant Cytochrome P450 CYP73 Expressed in Yeast.” European Journal of Biochemistry 224, no. 3 (September 15, 1994): 835–44. doi:10.1111/j.1432-1033.1994.00835.x.
    • Vancouver/ICMJE:
      Pierrel MA, Batard Y, Kazmaier M, Mignotte-Vieux C, Durst F, Werck-Reichhart D. Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast. European Journal of Biochemistry [Internet]. 1994 Sep 15 [cited 2020 Dec 2];224(3):835–44. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=hch&AN=12357405